Concentrating Stem Cell Therapy Naturally
Concentrating Stem Cell Therapy Naturally
By : Edward G. Loniewski, DO, FACOS, FAOAO
At Advanced Orthopedic Specialists we strive to provide you with cutting edge technology sometimes not even available at such stalwarts of medical innovation such as John Hopkins; The Mayo Clinic or even the University of Michigan. One of these advanced technologies involves the concept of concentrating the healing cells from your bone marrow or blood in the joint or area of damage. The simple filtering of your bone marrow or blood plasma to remove water and salts helps to naturally concentrate the important plasma proteins and growth factors found in this vital fluid found within each and every human. In the past, stem cell therapy involved spinning your bone marrow or blood at very high speeds to separate it into two to three different layers, namely the red blood cell layer, the platelet poor plasma (PPP) layer; and a platelet/ stem cell rich plasma ( PRP) layer. 
In the past, many researchers or physicians would discard both the red blood cell layer and the platelet poor plasma (PPP) layer. However, this never really made sense to me since this PPP layer contains many of the growth factors and plasma proteins necessary for continued growth of these cells. In fact, one of the best growth mediums for mesenchymal stem cells is a special mixture called platelet lysate made from the layer we would normally throw away in the biohazard bag. The problem using this PPP layer in the past is that it would dilute the mesenchymal stem cells as well as the concentrated platelets we need to activate in your body by forming a clump or a clot. However, some very smart and practical scientists at Celling Biosciences (Austin, Tx.) used good old fashioned American ingenuity to figure out a simple filtering process which can easily remove water and salts from this PPP to produce a super clot forming; growth factor and plasma protein rich environment for harvested stem cells. The concept is extremely simple and safe using no drugs or chemicals. This filtering process is a patented technology only available through a US born and bred company in Austin, Texas. Celling Bioscience's PhD's have used this self contained filter to significantly enhance the retention and growth of stem cells within your joints. In fact, they have demonstrated that this technology can increase the concentration of very vital growth factors such as Alpha 2 Macroglobulin (A2M) by a factor of 5.22 , Platelet Derived Growth Factor (PDGF-bb) by a factor of 5.88 , Vascular Endothelial Growth Factor (VEGF) by a factor of 2.27 as well as the very important Interleukin 1 Receptor Antagonist Protein (IRAP) by over 3.77 times the normal amount. In addition, this concentrating process helps the cells clump in a manner to protect them from migration to other parts of your body and slowly release themselves over a prolonged period of time. If there is no clumping of these cells within the area of delivery such as within your joint, you may rapidly lose these important cells within minutes.
Mesenchymal stem cells have a high affinity to blood vessels and travel to your spleen, lymph or circulatory system and away from the area of damage. Sometimes, stem cell clinics or researchers use artificial clotting agents such as human recombinant (meaning made from DNA taken from another human) or bovine (meaning from horses) thrombin to create this clot. The problem is that a percentage of patients react to this drug, and repetitive use of this drug can have an autoimmune response causing pain and swelling and possible cell death. On the other hand, you should never develop a reaction to a concentrate of your own cells. In a recent, trial conducted at Celling Biosciences, Matthew Murphy, PhD. was able to demonstrate that the super healthy clot formed from the concentrated plasma proteins allowed the clot to continue to hold it's shape for an amazing 14 days with very high cell counts. On the other hand, the clot formed by the drug thrombin had started to deteriorate only after 6 days with a much lower cell count. Dr. Loniewski, has worked with Celling Biosciences to help develop state of the art protocols using this technology and the most advanced stem cell therapies. No other center in the United States offers this advanced technology on all of their patients. This is why we can claim that at least 85% of our patients improve with little to no side effects. Not all patients respond to these treatments, and not every patient is a candidate for cell based therapies. If you are interested in cell based treatments, call our office at 810-299-8550 or you can download our on-line stem cell questionnaire to understand if you may be a candidate for this therapy.
